Supplier: SIGMA ALDRICH MICROSCOPY

Procion Red has been used in the visualization of osteocyte canalicular system. It has been used as an adsorbate in the biosorption by Bacillus subtilis.

Supplier: SIGMA ALDRICH MICROSCOPY

Indophenol Blue is used to colourimetrically measure N-containing compounds. The free amino acids react to form a blue color complex.

Supplier: SIGMA ALDRICH MICROSCOPY

Diethyl 3,4-pyridinedicarboxylate,97% (Cinchomeronic ester)

Supplier: SIGMA ALDRICH MICROSCOPY

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolouriser solution is a mixture of ethanol and acetone. Gram′s decolouriser solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolouriser dissolves the lipid layer from the gram-negative cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule. Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localisation of nucleic acids and lignin.

Supplier: SIGMA ALDRICH MICROSCOPY

This acid alcohol is part of Flourecent Stain Kit for Mycobacteria (Cat. No. 05151). It is used for decolorising.

Supplier: SIGMA ALDRICH MICROSCOPY

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).
Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water

Supplier: SIGMA ALDRICH MICROSCOPY

Staining system for polymorphonucleocytes in blood or bone marrow films.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

Supplier: SIGMA ALDRICH MICROSCOPY

This kit is intended for demonstration of nonspecific esterase in a subset of lymphocytes for determining maturation arrest. Kits 90A1 or 91A are intended for monocyte identification.

Supplier: SIGMA ALDRICH MICROSCOPY

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Supplier: SIGMA ALDRICH MICROSCOPY

May contain minor quantities of precipitated dye.

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

Supplier: SIGMA ALDRICH MICROSCOPY

Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol green is a sulfonephthalein dye. It is mainly used for measuring serum albumin. In acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630 nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 3.8) to blue color (5.4). It is widely used as a colloidal pigment.

Supplier: SIGMA ALDRICH MICROSCOPY

Morin hydrate is a yellowish pigment and a fluorescent indicator. It is used for the fluorimetric determination of several metals, mostly aluminum. Morin has been utilised in fluorescent microscopy as a fluorescent nuclear stain and as an application to detect aluminium in the brain by demonstrating metal salts.

Supplier: SIGMA ALDRICH MICROSCOPY

Counting leukocytes in the counting chamber.

Supplier: SIGMA ALDRICH MICROSCOPY

4-Amino-3-hydroxy-1-naphthalenesulfonic acid is a derivative of aniline consisting of a trifunctional monomer (bearing three functional groups –NH₂, –OH and –SO₃H). It also contains two fused benzene rings.

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Kit component for Sigma Alkaline Phosphatase Procedure, kits 86C and 86R

Supplier: SIGMA ALDRICH MICROSCOPY

Eriochrome Black T, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian blue 8GX, certified, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent diazoalkane useful as an HPLC derivatization reagent for carboxylic acid detection with better detection limit parameters and greater chemical stability than the commonly used 9-anthryldiazomethane.

Supplier: SIGMA ALDRICH MICROSCOPY

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

Supplier: SIGMA ALDRICH MICROSCOPY

Variamine Blue RT Salt has been used as a dye in ultraviolet-visible spectrophotometric method based-uricase enzyme for the detection of uric acid in normal urine. It is also used in combination with naphthol AS-MX phosphate-variam to determine the cytochemical visualization of alkaline phosphatase activity.

Supplier: SIGMA ALDRICH MICROSCOPY

Rose bengal,certified by the Biological Stain Commission, Dye content ≥80 % (4,5,6,7-Tetrachloro-2',4',5',7'-tetraiodofluorescein disodium salt; Acid Red 94; Bengal Rose B sodium salt; Rose Bengal sodium salt).

Supplier: SIGMA ALDRICH MICROSCOPY

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

Fast Garnet GBC base has been used as a stain in papain activity inhibition assay, to stain for tryptase-activity and chymase-like activity, to gel stain for esterolytic activity.

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin is mainly used as a diachrome and is part of the connective tissue stains. It also works as a pH indicator.