"PCR Mastermixes"

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: VWR Chemicals

VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Stemcell Technologies

For probe-based assays and arrays.

Supplier: VWR Chemicals

Fast HiFi Hot Start DNA Polymerase 2X Master Mix is a convenient alternative to Fast HiFi Hot Start DNA Polymerase, displaying the same set of features. Fast HiFi Hot Start DNA Polymerase 2X Master Mix is also available with a red dye for direct gel loading.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Supplier: Thermo Fisher Scientific

PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Supplier: EPPENDORF

Eppendorf Tubes® with snap caps are an ideal choice when working with medium-sized sample volumes (0,5 to 5,0 ml). Designed for user-friendly handling, broad temperature ranges, centrifugation safety (up to 25000×g), and sample recovery.

Supplier: EMBITEC

MasterMix PCR Taq 2X MiniOne 5x1ml (20µl per reaction) 1 * 1 KIT

Supplier: EMBITEC

MasterMix PCR FastTaq 2X MiniOne 5x1ml (20µl per reaction) 1 * 1 KIT

Supplier: EMBITEC

MiniLab PCR cycle number analysis. Includes lambda phage DNA template, FastTaq PCR MasterMix, forward and reverse primers, MiniOne D NA marker, MiniOne 5X sample loading dye, 0,2ml PCR tubes, 0,65ml microcentrifuge tubes, GreenGel cups (10), running 1 * 1 KIT

Supplier: PRIMERDESIGN

Real-time PCR Genotyping kit to quantify BRAF (V600E). Contains: Primer and multiplex probe mix - wildtype/variants, Positive contro ls - wildtype/variants, quasa mastermix 1 * 50 Tests

Supplier: PRIMERDESIGN

Real-time PCR Genotyping kit to quantify EGFR (T790M). Contains: Primer and multiplex probe mix - wildtype/variants, Positive contro ls - wildtype/variants, quasa mastermix 1 * 50 Tests

Supplier: EMBITEC

M6005: PCR Cycle Number Analysis MiniLab & Gel Electrophoresis Combo Reagent & Accessories for 10 Student Groups which includes: FastTaq PCR MasterMix (2X), Primer Set, Lambda DNA Sample, Sterile Nuclease-free Water, MiniOne DNA Marker, MiniOne 5X S 1 * 1 KIT

Supplier: PRIMERDESIGN

Real-time PCR Genotyping kit to quantify JAK2 (V617F). Contains: Primer and multiplex probe mix - wildtype/variants, Positive contro ls - wildtype/variants, quasa mastermix 1 * 50 Tests