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RNase A

Supplier: OMEGA BIO-TEK

Ribonuclease A (RNase A) is endonuclease-free and quality controlled for use in plasmid purification procedures for digestion of RNA.

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E.Z.N.A.® and E-Z 96® Cycle-Pure Kits

E.Z.N.A.® and E-Z 96® Cycle-Pure Kits

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Cycle-Pure kits are designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides, enzymes, salts, and other impurities from DNA samples. E.Z.N.A.® MicroElute Cycle-Pure kits are specifically intended to purify PCR samples with a small elution volume of 10 to 15 µl. The E-Z® 96 Cycle-Pure kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z® 96 Cycle-Pure kit utilises multiwell technology for manual or fully automated high throughput purification.

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E.Z.N.A.® Plasmid Mini kit II, Q-spin (without lid)

E.Z.N.A.® Plasmid Mini kit II, Q-spin (without lid)

Supplier: OMEGA BIO-TEK

This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

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E.Z.N.A.® Soil DNA kit

E.Z.N.A.® Soil DNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® soil DNA kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples. Isolated DNA can be used for most downstream applications including PCR, southern blotting, and SNP analysis.

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RNA isolation, E.Z.N.A.® HP total RNA kit

RNA isolation, E.Z.N.A.® HP total RNA kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

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E.Z.N.A.® blood DNA maxi kit

E.Z.N.A.® blood DNA maxi kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

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Plate sealing film, AeraSeal

Supplier: OMEGA BIO-TEK

AeraSeal is a 114 µm thick, hydrophobic porous film with medical-grade adhesive for tissue culture plates, bio-blocks, and 96-well plates where gas exchange is necessary for cell or bacterial growth.

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

DNA wash buffer concentrate, low salt spin column was solution (makes 500 ml)

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E.Z.N.A.® HP Plant & Fungal DNA Kit

E.Z.N.A.® HP Plant & Fungal DNA Kit

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal sample with organic solvents using spin columns.

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Plasmid isolation, single-strand phage DNA, M-13 isolation kits, E.Z.N.A.® and E-Z 96®

Supplier: OMEGA BIO-TEK

E.Z.N.A.® M13 DNA kits are designed to purify up to 10 μg of single-stranded DNA from up to 3 ml of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA kits are around 3 to 10 μg and reproducible when the isolations are performed from the same culture. Kits are also available in E-Z 96® DNA plate format.

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Ribonuclease A

Supplier: OMEGA BIO-TEK

Ribonuclease A

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Water nuclease-free

Supplier: OMEGA BIO-TEK

Water nuclease-free

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RNA isolation, total RNA kit, E.Z.N.A.® MicroElute

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

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MicroElute® Cycle-Pure & Gel Extraction Kit, Omega Bio-tek

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

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E.Z.N.A.® Plant DNA DS kit

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

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Tissue DNA kit, genomic DNA isolation from tissue, E.Z.N.A.®

Tissue DNA kit, genomic DNA isolation from tissue, E.Z.N.A.®

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

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