28227 Results for: "G-Biosciences"

Supplier: G-Biosciences

FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

Supplier: G-Biosciences

Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Supplier: G-Biosciences

SilverOUT removes interfering silver ions from gel pieces.

Supplier: G-Biosciences

UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.

Supplier: G-Biosciences

HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.

Supplier: G-Biosciences

Yeast Geno-DNA Template™ isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitation and centrifugation.

Supplier: G-Biosciences

G-Biosciences' FASTsilver™ is a rapid silver stain for nucleic acids and proteins in electrophoresis gels

Supplier: G-Biosciences

These cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulphydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules. Functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls, carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents.

Supplier: G-Biosciences

A selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure.

Supplier: G-Biosciences

The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

Supplier: G-Biosciences

Cetrimonium bromide

Supplier: G-Biosciences

LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualisation.

Supplier: G-Biosciences

Sodium deoxycholate

Supplier: G-Biosciences

This book provides scientists some basic tools for managing the patent rights of their research and provides a working knowledge of patent procedures useful for the fast-paced research environment. After reading this book scientists will be familiar with the basic steps necessary to obtain patent rights to their inventions.

Supplier: G-Biosciences

Anti-IgG Goat Antibody (AP (Alkaline Phosphatase))

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

Supplier: G-Biosciences

Ethylene glycol bis(succinimidyl succinate) Homobifunctional Cross Linker.

Supplier: G-Biosciences

N-γ-Maleimidobutyryloxysulfosuccinimide ester Heterobifunctional Cross Linker.

Supplier: G-Biosciences

Ideal for enzyme-antibody crosslinking.

Supplier: G-Biosciences

Iodoacetamide

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

Supplier: G-Biosciences

Lithium acetate 1 mol/l in aqueous solution

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

Nucleic Acid Purification Kits and Reagents, G-CAPSULE™ Weight, to prevent G-CAPSULE™ from floating during electroelution

Supplier: G-Biosciences

Magnesium chloride solution 1 M

Supplier: G-Biosciences

HOOK™-PEG₂-Iodoacetyl-biotin, 50 mg

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.