28227 Results for: "G-Biosciences"

Supplier: G-Biosciences

(3-((3-Cholamidopropyl)dimethylammonio)-2-hydroxy-1-propanesulphonate)

Supplier: G-Biosciences

Deoxy-big CHAP (N,N-bis[3-(D-gluconamido)propyl]deoxycholamide)

Supplier: G-Biosciences

N-Octyl-β-D-glucopyranoside

Supplier: G-Biosciences

Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Supplier: G-Biosciences

Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

Supplier: G-Biosciences

Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.

Supplier: G-Biosciences

G-Biosciences offers several different biotechnology-grade agarose powders. G-Biosciences' Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. It is suitable for nucleic acid analytical and preparative electrophoresis, blotting, and protein electrophoresis (i.e. radial immunodiffusion).

Supplier: G-Biosciences

TEMED (N,N,N’,N’-Tetramethylethylenediamine) is a 99,9% pure polymerisation reagent used when making polyacrylamide gels used in gel electrophoresis.

Supplier: G-Biosciences

Potassium chloride

Supplier: G-Biosciences

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Supplier: G-Biosciences

L(+)-Lysine monohydrochloride

Supplier: G-Biosciences

Orthoboric acid

Supplier: G-Biosciences

Pig Native Gelatin (from Skin)

Supplier: G-Biosciences

This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Supplier: G-Biosciences

Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

Supplier: G-Biosciences

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: G-Biosciences

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

Supplier: G-Biosciences

Brij® 58

Supplier: G-Biosciences

InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

Supplier: G-Biosciences

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

Supplier: G-Biosciences

Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, in vitro translation, RNase protection assays and poly (A+) selection.

Supplier: G-Biosciences

CytoScan™-SRB Cytotoxicity Assay is based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB). The assay is used for cell density determination, based on the measurement of cellular protein content.

Supplier: G-Biosciences

After Coomassie Brilliant Blue staining process, the band intensity may be further enhanced by de-staining the stained gel in our CBB De-Staining Solution.

Supplier: G-Biosciences

DNAmark™ 500 bp ladder consists of 10 DNA fragments ranging from 500 bp to 5000 bp. The 2000 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 2000 bp are 40 ng. The 2000 bp fragment is 100 ng.

Supplier: G-Biosciences

Potassium acetate

Supplier: G-Biosciences

Ammonium peroxodisulphate (APS), powder

Supplier: G-Biosciences

This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.

Supplier: G-Biosciences

Sodium dodecyl sulphate (SDS) 200 g/l in aqueous solution