illustra™ GFX™ PCR DNA and gel band purification kit
Supplier: Cytiva
The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
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Percoll™ PLUS
Supplier: Cytiva
Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.
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Empty columns, XK series
Supplier: Cytiva
XK empty columns are designed for liquid chromatography at low to medium pressure. The user friendly design ensures trouble-free operation with fittings for direct connection to high performance liquid chromatography systems. They are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules. They are not resistant to acetone, ktones, chlorinated hydrocarbons, aliphatic esters and phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid and strong mineral acids.
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Accessories for ÄKTA chromatography systems
Supplier: Cytiva
Screw lid kit, includes PP screw lid and EPDM cap membrane
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illustra™ MicroSpin™ S-400 HR columns
Supplier: Cytiva
illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.
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Blotting membranes, Amersham™ Hybond™-XL
Supplier: Cytiva
Amersham™ Hybond™-XL is a positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labelled probes. Amersham™ Hybond™-XL has a greater concentration and a more even distribution of positive charge than other charged nylon membranes. It retains all the features of other nylon membranes, including high binding capacity up to 600 µg/cm², efficient binding of small fragments, effective probe removal and exceptional properties for reprobing.
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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Blotting membranes, Amersham™ Hybond™-N+
Supplier: Cytiva
Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.
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Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Ficoll-Paque® PLUS and Ficoll-Paque® PREMIUM Centrifugation Media, Cytiva
Supplier: Cytiva
Ficoll-Paque™ PREMIUM products are a range of sterile, ready to use density gradient media for the preparation of mononuclear cells.
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illustra™ ExoProStar™ 1-STEP kits
Supplier: Cytiva
illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.
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Sera-Mag carboxylate-modified magnetic beads and SpeedBeads
Supplier: Cytiva
Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH2).
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Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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illustra™ ProbeQuant™ G-50 micro columns
Supplier: Cytiva
ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
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Affinity chromatography columns, HisTrap™ FF
Supplier: Cytiva
HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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Affinity chromatography columns, HiTrap™ NHS-Activated HP
Supplier: Cytiva
HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.
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Size exclusion chromatography columns, illustra™ NAP™
Supplier: Cytiva
NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.
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Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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PCR beads, illustra™ PuReTaq Ready-To-Go™
Supplier: Cytiva
PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.
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Affinity chromatography columns, HisTrap™ excel
Supplier: Cytiva
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
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HiLoad Superdex columns, 200 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Small-scale SEC columns, Superose 6 Increase
Supplier: Cytiva
Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.
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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Accessories for chromatography systems
Supplier: Cytiva
LabMate PD-10 buffer reservoir
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Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
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illustra™ MicroSpin™ G-25 columns
Supplier: Cytiva
For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.
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Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
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Polyribonucleotide poly(I)·poly(C)
Supplier: Cytiva
The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.
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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.