2105 Results for: "Cytiva"
illustra™ TempliPhi™100/500 Amplification Kits
Supplier: Cytiva
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
Expand 2 Items
Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
Expand 3 Items
Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
Expand 1 Items
Magnetic rack, MagRack™ 6
Supplier: Cytiva
MagRack 6 is a magnetic rack for small-scale protein purification and sample enrichment with magnetic beads. Each rack consists of anodized aluminium housing (blue), detachable plastic bar (white) containing a neodymium magnet.
Expand 1 Items
Amersham™ Protran® Premium Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® Premium nitrocellulose blotting membranes provide excellent sensitivity, resolution and low background in fluorescence Western blotting detection applications.
Expand 15 Items
Size exclusion chromatography columns, Superdex™ 75 Increase
Supplier: Cytiva
Superdex 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion chromatography applications.
Expand 3 Items
Film, Amersham™ Hyperfilm™ ECL™
Supplier: Cytiva
Amersham™ Hyperfilm™ ECL™ is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer. The anti-scratch coating reduces the risk of visible artefacts (e.g., plastic wrap marks) and the clear colourless base results in easy reading of images without compromising on sensitivity.
Expand 5 Items
Cation exchange chromatography columns, HiTrap SP HP
Supplier: Cytiva
HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification.
Expand 3 Items
Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: Cytiva
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Expand 4 Items
Cation exchange chromatography columns, HiTrap™ SP FF
Supplier: Cytiva
HiTrap™ SP FF is pre-packed with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions.
Expand 2 Items
Precast ExcelGel for SDS-PAGE
Supplier: Cytiva
ExcelGel precast gels for SDS-PAGE are available as homogeneous or gradient gels. The homogeneous gels have 25 preformed sample wells for sample volumes from 5 to 17 µl. The gradient gels use sample application strips (26 samples up to 40 µl, 52 samples up to 20 µl), paper pieces or sample droplets. All ExcelGel SDS homogeneous and 8 - 18 gradient gels have a 33 mm stacking zone and a 77 mm separating zone.
Expand 3 Items
DNA clean up, Sephadex® G-50 DNA Grade F
Supplier: Cytiva
Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.
Expand 2 Items
Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
Expand 1 Items
Desalting columns with Sephadex G-25 resin, HiTrap™
Supplier: Cytiva
HiTrap Desalting are ready-to-use 5 ml columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
Expand 3 Items
Amersham™ Protran® Supported Nitrocellulose Western Blotting Membranes, Cytiva
Supplier: Cytiva
Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.
Expand 11 Items
Affinity chromatography columns, HisTrap™ HP
Supplier: Cytiva
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
Expand 5 Items
Gel filtration media, Sephadex™ G-50
Supplier: Cytiva
Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
Expand 5 Items
Western blotting detection reagent, Amersham™ ECL Select™
Supplier: Cytiva
Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
Expand 1 Items
Anion exchange chromatography columns, Mono Q™
Supplier: Cytiva
Mono Q™ columns are strong anion exchange chromatography columns for protein analysis or small scale, high resolution polishing of proteins.
Expand 1 Items
Desalting columns, HiPrep™
Supplier: Cytiva
HiPrep™ 26/10 desalting column is a convenient, easy to use column pre-packed with Sephadex™ G-25 Fine for desalting and buffer exchange with sample sizes of up to 15 ml per column.
Expand 2 Items
Affinity chromatography columns, His GraviTrap™
Supplier: Cytiva
His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
Expand 1 Items
Anti-IgG Sheep Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
Expand 1 Items
Sample concentrators, Vivaspin®
Supplier: Cytiva
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.
Expand 24 Items
Columns, PD MidiTrap™ G-25
Supplier: Cytiva
PD MidiTrap G-25 is convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, The use of centrifugation protocol with all gravity columns.
Expand 1 Items
Affinity chromatography columns, HiTrap™ protein G HP
Supplier: Cytiva
HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Expand 5 Items
Empty disposable columns, PD-10
Supplier: Cytiva
PD-10 Columns are economical, plastic columns for gravity flow purification.
Expand 1 Items
Empty columns, XK series
Supplier: Cytiva
XK empty columns are designed for liquid chromatography at low to medium pressure. The user friendly design ensures trouble-free operation with fittings for direct connection to high performance liquid chromatography systems. They are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules. They are not resistant to acetone, ktones, chlorinated hydrocarbons, aliphatic esters and phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid and strong mineral acids.
Expand 12 Items
Western blotting blocking reagent, Amersham ECL™ Prime
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
Expand 1 Items
Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
Expand 2 Items
illustra™ GFX™ PCR DNA and gel band purification kit
Supplier: Cytiva
The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.