"Buffer Solution - Blue"

Experimentation kits, assays for protein quantification

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

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Hemacolor® Rapid staining of blood smear kit stain for hematology, for histology, Sigma-Aldrich®

Supplier: Merck

For use in hematology and clinical cytology

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pH indicator papers, MQuant®, Supelco®

Supplier: Merck

Traditional pH indicator test papers, supplied on a 4,8 metre roll, consisting of high quality filter paper impregnated with indicator and mixed indicator solutions then dried and cut to size.

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Trypan blue solution 0,4% (w/v) in PBS stain for histology pH 7,5 ±0,5, Corning®

Supplier: Corning

Trypan blue is the most common stain used in cell count and viability assays.

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Vented Millex® GS Syringe Filter

Supplier: Merck

Vented Millex®-GS filter units are syringe filters for sterilisation of low volume aqueous solutions where venting is desired.

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VWR® Fluorometer

Supplier: VWR Collection

VWR® Fluorometer for quantitation of DNA, RNA, and Proteins. Compact with touch screen control panel. Compatible with VWR® quantitation kits and kits from leading suppliers.

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Luminaris color Probe qPCR master mixes

Supplier: Thermo Fisher Scientific

Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

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Luminaris color HiGreen qPCR master mix

Supplier: Thermo Fisher Scientific

Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.