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PCR cloning kit, CloneJET

PCR cloning kit, CloneJET

CloneJET PCR Cloning Kit, 20 reactions, 1 KIT
Catalog # K1231
Supplier:   Thermo Fisher Scientific
PCR cloning kit, CloneJET
Catalog # K1231
Supplier:  Thermo Fisher Scientific
CAS Number:  
PCR cloning kit, CloneJET
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PCR cloning kit, CloneJET

CloneJET PCR Cloning Kit, 20 reactions, 1 KIT

Specifications
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Specifications
CAS Number:  
Price
1.580,00 kr
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1 * 1 Each
Product Details & Documents

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Specifications

Specifications

  • Pk:
    1 KIT
  • Description:
    CloneJET PCR Cloning Kit, 20 reactions

Specifications

   
VWR®, Nitrile Gloves

  #: 112-2373

Supplier: VWR Collection

Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

  #: 83639.320

Supplier: VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

  #: 20864.320

Supplier: VWR Chemicals

VWR®, Transfer Pipettes, High Performance

  #: 612-1681

Supplier: VWR Collection

Acetone ≥99%, TECHNICAL

  #: 20063.365

Supplier: VWR Chemicals

VWR® Serological Pipette, Standard Line

  #: 612-3700

Supplier: VWR Collection

VWR® NITRILE LIGHT, Nitrile Gloves

  #: 112-2756

Supplier: VWR Collection

Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83640.320

Supplier: VWR Chemicals

VWR® Facial Tissues

  #: 115-0600

Supplier: VWR Collection

Nitrile gloves, Kimtech™ Purple Nitrile™

  #: 112-4847

Supplier: Ansell

Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

  #: 1.00030.2500

Supplier: Merck

pH indicator strips, MQuant®, Supelco®

  #: 1.09535.0001

Supplier: Merck

Sealing film for manual application, Parafilm® M

  #: 291-0057

Supplier: HEATHROW

Water, HiPerSolv CHROMANORM® for HPLC

  #: 23595.328

Supplier: VWR Chemicals

Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

  #: 31770.294

Supplier: VWR Chemicals

VWR® AVS TITRINORM Buffer Solutions, 20 °C

  #: 32096.291

Supplier: VWR Chemicals

Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83645.320

Supplier: VWR Chemicals

Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

  #: 1.09439.1000

Supplier: Merck

VWR® Nitrile Extra Light, Nitrile Gloves

  #: 112-4195

Supplier: VWR Collection

epT.I.P.S.® Bulk - Pipette Tips

  #: 613-3508

Supplier: EPPENDORF