Hybridisation buffers

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Denhardt solution is suitable for nucleic acid hybridisation. Denhardt's solution is a mi×ture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. It consists of 2% Ficoll 400, 300mM NaCl, 2% polyvinylpyrrolidone, and 2% BSA.
The SSPE or or Saline-Sodium phosphate-EDTA buffer is a general hybridisation buffer that consists of 0,2 M phosphate buffer (at a pH of 7,4), 2,98 M NaCl, and 0,02 M EDTA.
The TMAC hybridisation Solution consists of 3 M Tetramethyl ammonium chloride, 50mm Tris-HCl (at a pH of 8,0), 1 mm EDTA (at a pH of 8,0), and 0,1% SDS.
The TNT Buffer is a general molecular biology and hybridisation buffer that consists of 0,1 M Tris-HCl (at a pH of 7,5), 0,15 M NaCl, and 0,05% Tween®-20.