A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.  Contains all Oxford Nanopore sequencing reagents for rapid whole-genome sequencing of SARS-CoV-2.

• Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA

• Rapid Barcoding Kits 96 V14 to barcode and prepare the amplified material for sequencing
• MinION/GridION Flow Cells to perform sequencing on MinION or GridION devices

The COVID Mini V14 bundle contains: 
- 1× Midnight Expansion Kit (EXP-MRT001)
- 2× Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
- 6× MinION/GridION Flow Cells (FLO-MIN114)

The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopore's rapid chemistry can be deployed, enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turnaround times that can assist with monitoring of novel variants of concern.

The protocol delivers robust full-genome coverage across a wide range of Ct values.

Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow. The workflow and bundle are designed to run 96 samples at a time. Users wishing to run in multiples of 24 will need to buy 3× Flow Cell Wash Kits (EXP-WSH004).

Sample multiplexing: ≤96

Flow cells and kits are shipped together at 2 to 8 °C. The packaging is designed to protect the flow cells from freezing. R10.4.1 Flow Cells can be stored unopened at 2 to 8 °C for 12 weeks or unopened at room temperature for one month. Kits should be stored at ‒20 °C. Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.