NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

• Bi-functional substrates
• Rapid, no-wash assay for microscopy or flow cytometry
• Stable for several days for performing time-course assays
• Tolerant of formaldehyde fixation and permeabilisation
• Use in adherent or suspension cell cultures
• For flow cytometry, microscopy, live cell imaging, or microplate readers

NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. Upon entering apoptotic cells, the non-fluorescent substrate is cleaved by caspases-3/7 releasing a high-affinity DNA binding dye which migrates to the nucleus and becomes fluorescent upon binding to DNA.

NucView® 488 caspase-3 substrate is offered as a 1 mM solution in PBS (phosphate-buffered saline) for cells that are sensitive to DMSO toxicity. NucView® 405 and 530 are available in 1 mM in PBS and trial sizes are available for both PBS and DMSO formulations.