Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

• Detection method: Chemiluminescence
• Antibody stripping solution (10X) - (1 container, 50 ml)
• Blocking buffer (20X)
• Milk-based - (1 container, 50 ml)

The MILLIPORE re-blot western blot recycling kit is effective for removal of antibodies from western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, and more.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

The re-blot western blot recycling kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.