Immunomagnetic positive selection of particle-free human cells labelled with biotinylated antibodies.

• Highly purified cells labelled with biotinylated antibodies isolated from human tissue in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™

The EasySep™ Release Human Biotin Positive Selection Kit is designed to isolate cells that are labelled with biotin-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labelled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, Biotin-antibody labelled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. This product replaces the EasySep™ Human Biotin Positive Selection Kit, providing highly purified particle-free cells.