Immunomagnetic positive selection of human CD34+ cells (e.g. hematopoietic, stem/progenitor cells)
- Fast and easy-to-use
- Up to 99% purity
- No columns required
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognising CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimise nonspecific binding. labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.
This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations. For large-scale isolation of human CD34+ cells from mobilized leukopaks, see the large-format (1×10^10 cells) kit (Catalog #100-1569).