RSeT™ Medium (2-Component)

STMC05978

: STEMCELL Technologies

STMC05978

: STEMCELL Technologies

Pk:
1 KIT
Description:
RSeT™ Medium (2-component)

Defined medium for naïve-like human pluripotent stem cells.

RSeT™ Medium is a defined cell culture medium used for the reversion of primed human pluripotent stem cells (hPSCs) to a naïve-like state and maintenance of naïve-like hPSCs under feeder-dependent and hypoxic conditions. RSeT™ Medium contains pre-screened quality components and does not contain bFGF or TGFβ. It is compatible with human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. hPSCs cultured in RSeT™ Medium exhibit features of a naïve-like state such as tightly packed, domed colonies with refractive edges. Key transcripts associated with naïve-like hPSCs such as KLF2, KLF4, and TFCP2L1 show increased expression in hPSCs cultured in RSeT™ Medium. RSeT™ hPSCs can be converted back to a primed state by culture in mTeSR™1 and can then be differentiated with STEMdiff™ products such as STEMdiff™ Definitive Endoderm Kit, STEMdiff™ SMADi Neural Induction Kit, and STEMdiff™ Mesoderm Induction Medium.

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