Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN appears to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis

Applications include promotion of cell attachment and spreading, rapid expansion of cell populations, serum-free or reduced serum culture, cell adhesion assays, studies of the effects of fibronectin on cell behaviour, and improving survival of primary cells in culture.