Human Recombinant FGF-basic 147 (Animal free) (from E. coli)

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic. Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.

High Quality
Low Endotoxin
Affordable

A COA is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions. This product is for research use only and is not for use in humans.

: For research use only.

Conjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
–20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤0,1
Biological activity:
Bioactivity assay: NR6R-3T3 cell proliferation, ED50 ≤5 ng/ml, Bioactivity ≥2,0×10⁵ units/mg
Gene ID:
P09038
Reconstitution instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
Y
Protein synonyms:
Prostatropin|HBGF-2|FGF2
Protein/peptide name:
FGF-basic 147
Purity:
≥95%
Molecular weight:
16,5 kDa
Sequence:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Endotoxin level:
Low
Formulation:
10 mM sodium phosphate, 75 mM sodium chloride, pH 7,5
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay