Leupeptin hydrochloride, MP Biomedicals
About this item
A modified tripeptide reversible protease inhibitor of trypsin-like proteases and some cysteine proteases including endoproteinase Lys-C, kallikrein, papain, thrombin, cathepsin B, cathepsins H and L, trypsin, calpain, trypsin and plasmin. Little to no inhibition is seen against pepsin, cathepsins A and D and alpha-chymotrypsin. When adjusted for molarity, all three salt forms are equally effective; however, the hydrochloride salt is usually less invasive in biological settings. Leupeptin, because of its aldehyde group, may act as a reducing agent and therefore interfere in protein determinations such as Lowry and, to a lesser extent, Bradford.
- White to off-white powder
- Store at -20 °C; desiccate; protect from light
Leupeptin hydrochloride is used in immunoblotting and acts as a protease inhibitor. Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined by HPLC should take into account the three main peaks. Majority of contaminating peptide is racemized leupeptin. The primary mechanism of inactivation is racemization of the L-arginal; the D-arginal form is totally inactive. If the aldehyde is oxidized but retains its L-configuration, the resulting compound does have some inhibitory activity.
4 Options Available Below
Product Details & Documents
A modified tripeptide reversible protease inhibitor of trypsin-like proteases and some cysteine proteases including endoproteinase Lys-C, kallikrein, papain, thrombin, cathepsin B, cathepsins H and L, trypsin, calpain, trypsin and plasmin. Little to no inhibition is seen against pepsin, cathepsins A and D and alpha-chymotrypsin. When adjusted for molarity, all three salt forms are equally effective; however, the hydrochloride salt is usually less invasive in biological settings. Leupeptin, because of its aldehyde group, may act as a reducing agent and therefore interfere in protein determinations such as Lowry and, to a lesser extent, Bradford.
- White to off-white powder
- Store at -20 °C; desiccate; protect from light
Leupeptin hydrochloride is used in immunoblotting and acts as a protease inhibitor. Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined by HPLC should take into account the three main peaks. Majority of contaminating peptide is racemized leupeptin. The primary mechanism of inactivation is racemization of the L-arginal; the D-arginal form is totally inactive. If the aldehyde is oxidized but retains its L-configuration, the resulting compound does have some inhibitory activity.
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- ICNA0219562401
- ICNA0219562405
- ICNA0219562425
- ICNA0219562480
- Protein/peptide name:
- Leupeptin hydrochloride
- Leupeptin hydrochloride
- Leupeptin hydrochloride
- Leupeptin hydrochloride
- Protein synonyms:
- Acetyl-Leu-Leu-Arg-al hydrochloride
- Acetyl-Leu-Leu-Arg-al hydrochloride
- Acetyl-Leu-Leu-Arg-al hydrochloride
- Acetyl-Leu-Leu-Arg-al hydrochloride
- CAS No.:
- 24125-16-4
- 24125-16-4
- 24125-16-4
- 24125-16-4
- Purity:
- 60%
- 60%
- 60%
- 60%
- Colour:
- White to off-white
- White to off-white
- White to off-white
- White to off-white
- Molecular weight:
- 463.02
- 463.02
- 463.02
- 463.02
Specifications
Product Family Options
Product Information
- Pack typePkAvailabilityPrice
- Specifications:
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Specifications
Cat. No.ICNA0219562401Protein/peptide nameLeupeptin hydrochlorideProtein synonymsAcetyl-Leu-Leu-Arg-al hydrochlorideCAS No.24125-16-4Purity60%ColourWhite to off-whiteMolecular weight463.02 - Specifications:
More
Specifications
Cat. No.ICNA0219562405Protein/peptide nameLeupeptin hydrochlorideProtein synonymsAcetyl-Leu-Leu-Arg-al hydrochlorideCAS No.24125-16-4Purity60%ColourWhite to off-whiteMolecular weight463.02 - Specifications:
More
Specifications
Cat. No.ICNA0219562425Protein/peptide nameLeupeptin hydrochlorideProtein synonymsAcetyl-Leu-Leu-Arg-al hydrochlorideCAS No.24125-16-4Purity60%ColourWhite to off-whiteMolecular weight463.02 - Specifications:
More
Specifications
Cat. No.ICNA0219562480Protein/peptide nameLeupeptin hydrochlorideProtein synonymsAcetyl-Leu-Leu-Arg-al hydrochlorideCAS No.24125-16-4Purity60%ColourWhite to off-whiteMolecular weight463.02
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck