tetranor-PGDM ELISA kit
Supplier: Cayman Chemical
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About this item
A competitive assay for the quantification of tetranor-PGDM in urine.
- Detect tetranor-PGDM, a major metabolite of PGD₂ in urine
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure tetranor-PGDM levels down to 40 pg/ml
- Read: Colorimetric at 405 to 420 nm
tetranor-PGDM is a major metabolite of prostaglandin D2 (PGD₂) found in human and mouse urine. In human urine, tetranor-PGDM is significantly more abundant than the PGD2 metabolites 11β-PGF2α and 2,3-dinor-11β-PGF₂α and is the only endogenous PGD₂ metabolite detectable in mouse urine by LC-MS.
Normal levels of tetranor-PGDM in human and mouse urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively. tetranor-PGDM ELISA Kit is a competitive assay that can be used for quantification of tetranor-PGDM in urine. This assay converts tetranor-PGDM to a stable derivative, tetranor-PGJM, that can be easily quantified.
Packaging: Kit contains: Antiserum, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover sheet, Ellman's reagent, tracer and antiserum dye.
Specifications
- Developmental time:60 - 90 min
- Mid point:120 - 250 pg/ml (50% B/B₀)
- Detection range:6,4 - 4000 pg/ml
- Storage temperature:–20 °C
- Sensitivity:40 pg/ml (80% B/B₀)
- Incubation time:18 hr
Specifications
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