Direct cGMP ELISA kit

ENZOADI900014

Direct cGMP ELISA kit

ENZOADI900014

Pk:
1 KIT
Assay Type:
Direct
Target protein:
cGMP
Description:
Direct cGMP ELISA kit
Seq:
02
Sensitivity:
(non-acetylated) 0,604 pmol/ml; (acetylated) 0,025 pmol/ml (range (non-acetylated) 0,8 - 500 pmol/ml; (acetylated) 0,08 - 50 pmol/ml)
Tests per kit:
96 tests

Most sensitive (0,025pmol/m) colorimetric kit for intracellular cGMP quantification.

For the quantitative determination of cGMP in cell lysates and tissue from any species. Cited sample types include cerebral spinal fluid and microdialysate.

This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.

: Contains GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine. Alternative size available: 89141-284 (5x96 wells).