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ROS-ID™ total ROS/superoxide detection kit
ROS-ID™ total ROS/superoxide detection kit
Catalog # ENZOENZ51010
Supplier:  ENZO LIFE SCIENCES
CAS Number:  
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ROS-ID™ total ROS/superoxide detection kit
Catalog # ENZOENZ51010
Supplier:  ENZO LIFE SCIENCES
CAS Number:  

Specifications

  • Pk:
    1 KIT
  • Description:
    ROS-ID® Total ROS/Superoxide detection kit
  • Seq:
    02
  • Storage conditions:
    −80 °C
  • Application:
    Flow cytometry, fluorescence microscopy, fluorescent detection and HTS
  • Size:
    1 kit

Specifications

About this item

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

  • Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals
  • High sensitivity, specificity and accuracy for live cell studies
  • Compatible with major components of tissue culture media (phenol red, FBS and BSA)
  • Complete set of reagents, including ROS inducers and scavengers
  • Validated on microscopy, flow cytometry, and microplates

Through the combination of these two specific fluorescent probes, the kit provides a simple and specific assay for the real-time measurement of global levels of reactive oxygen species (ROS), and specifically superoxide in living cells. The Total ROS/Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension) or 2 x 96 microplate assays.

Delivery: Kit contains Oxidative Stress Detection Reagent (Green), 300 nmoles, Superoxide Detection Reagent (Orange), 300 nmoles, ROS Inducer (Pyocyanin), 1 µmole, ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg and Wash Buffer Salts, 1 pack.