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Human recombinant IL15 (from E. coli)
Human recombinant IL15 (from E. coli)
Catalog # PRSI91-013
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant IL15 (from E. coli)
Catalog # PRSI91-013
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Protein/peptide type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Environmentally Preferable:
  • Storage conditions:
    Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
  • Biological activity:
    ED50 is less than 0.5 ng/ml as determined by the dose dependent stimulation of mouse CTLL-2 cell proliferation.
    Specific Activity of 2.0 x 106 IU/mg.
  • Protein synonyms:
    IL15|Interleukin-15|IL-15
  • Protein/peptide name:
    IL15
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular weight:
    12.5 kD
  • Sequence:
    Asn49-Ser162
  • Formulation:
    Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

Human Interleukin 15 (IL-15) is a cytokine that regulates T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL15 receptor (IL-15RA) with high affinity. IL-15 also binds to the beta and gamma chains of the IL-2 receptor, but not the alpha subunit of the IL2 receptor. IL-15 is structurally and functionally related to IL-2. Both cytokines share some subunits of receptors, allowing them to compete for and negatively regulate each other's activity.

  • This recombinant protein can be used for biological assays. For research use only.

The number of CD8+ memory T cells is controlled by a balance between IL-15 and IL-2. Despite their many overlapping functional properties, IL-2 and IL-15 are, in fact, quite distinct players in the immune system. IL-15 is constitutively expressed by a wide variety of cell types and tissues, including monocytes, macrophages and DCs. Mature Human IL-15 shares 70% amino acid sequence identity with Mouse and Rat IL-15.