These protein-based columns are suitable for separation of acidic and non-protolytic compounds. These columns function entirely in the reversed phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values.

CHIRALPAK® HSA is narrower applicability than CHIRALPAK® AGP. Separates preferentially weak and strong acids and non-protolytic compounds.

The chiral selector in this stationary phase is human serum albumin (HSA). The protein has been immobilised onto spherical 5 μm particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatisation. The column is operated in the reverse phase mode. With the CHIRALPAK® HSA column, both racemic acids and amino acids can be resolved directly, without derivatisation.