Brain-derived Neurotrophic Factor (BDNF) Rapid™ ELISA Kit (CE marked)

The Biosensis CE Marked BDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards.

The BDNF ELISA Kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognise the mature as well as the pro-form of BDNF. However, cross-reactivity to the full-length proBDNF protein is low.

: The CE Marked BDNF Rapid™ ELISA Kit is approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. (www.calbiotech.com) for Biosensis.

: Kit contains BDNF antibody coated 96 well microplate strips, Assay Diluent A (ready to use), Recombinant BDNF Standard (freeze-dried), BDNF Detection antibody (ready to use), Streptavidin-HRP (ready to use), Wash Buffer (20X), TMB Substrate (1X), Stop Solution (1X), and plate sealer.

Assay Type:
Sandwich
Primary antibody reactivity:
Human
Target protein:
BDNF
Cross reactivity:
Reacts with BDNF from mouse, rat and numerous other species due to BDNF sequence homology
Detection range:
7,8 - 500 pg/ml
Storage temperature:
2...8 °C
Sensitivity:
Typical limit of detection (LOD) for BDNF is < 3 pg/mL determined by calculating the mean plus 2× standard deviation of mean of blank (n=20)