Lymphocyte separation medium, LYMPHOSEP® is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinised or defibrinated human blood.

• In vitro isolation of lymphocytes from diluted whole blood
• Maximum yield of mononuclear cells
• Easy one-step centrifugation
• >96% cell viability of lymphocytes
• Low endotoxins
• Autoclave sterilization

Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma LymphoSep interface.