This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

• No reducing or chelating agents
• Designed for room temperature storage
• Fast lysis completes after 20 minutes hands-off incubation
• Requires no rigorous mechanical disruption or lengthy enzymatic treatments

The reagent is used for Western blots, GST and histidine-tagged purification based applications. This fully dialysable formulation, allows complete exchange of the extracted protein into any buffer required by downstream techniques. The tris-based buffer has a very low ionic strength and does not include sodium chloride. The reagent is compatible with both the Thermo Scientific Pierce BCA and Coomassie Plus Protein Assays.