Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: rat

About this item

The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.

This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.

This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications.

Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples.

Delivery: The ELISA kit box contains 1 or 2×96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

Caution: It has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Specifications

Assay Type:
Sandwich
Primary antibody reactivity:
Rat
Target protein:
GDNF
Cross reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Specificity:
Rat. The antibodies used in this kit detect mouse and human GDNF.
Detection range:
7,8 – 500 pg/ml
Sensitivity:
Typical limit of detection (LOD) for rrGDNF is <15 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10).