Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: mouse

The Biosensis GDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular mouse serum samples.

This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.

This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications.

The kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards.

: The ELISA kit box contains 1 or 2×96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

: It has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Assay Type:
Sandwich
Primary antibody reactivity:
Mouse
Target protein:
GDNF
Cross reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF.
Detection range:
7,8 – 500 pg/ml
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is <10 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10).