Hybridisation buffers
About this item
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
Denhardt solution is suitable for nucleic acid hybridisation. Denhardt's solution is a mi×ture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. It consists of 2% Ficoll 400, 300mM NaCl, 2% polyvinylpyrrolidone, and 2% BSA.
The SSPE or or Saline-Sodium phosphate-EDTA buffer is a general hybridisation buffer that consists of 0,2 M phosphate buffer (at a pH of 7,4), 2,98 M NaCl, and 0,02 M EDTA.
The TMAC hybridisation Solution consists of 3 M Tetramethyl ammonium chloride, 50mm Tris-HCl (at a pH of 8,0), 1 mm EDTA (at a pH of 8,0), and 0,1% SDS.
The TNT Buffer is a general molecular biology and hybridisation buffer that consists of 0,1 M Tris-HCl (at a pH of 7,5), 0,15 M NaCl, and 0,05% Tween®-20.
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Product Details & Documents
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
Denhardt solution is suitable for nucleic acid hybridisation. Denhardt's solution is a mi×ture of high-molecular weight polymers capable of saturating non-specific binding sites and artificially increasing the concentration of available probe. It consists of 2% Ficoll 400, 300mM NaCl, 2% polyvinylpyrrolidone, and 2% BSA.
The SSPE or or Saline-Sodium phosphate-EDTA buffer is a general hybridisation buffer that consists of 0,2 M phosphate buffer (at a pH of 7,4), 2,98 M NaCl, and 0,02 M EDTA.
The TMAC hybridisation Solution consists of 3 M Tetramethyl ammonium chloride, 50mm Tris-HCl (at a pH of 8,0), 1 mm EDTA (at a pH of 8,0), and 0,1% SDS.
The TNT Buffer is a general molecular biology and hybridisation buffer that consists of 0,1 M Tris-HCl (at a pH of 7,5), 0,15 M NaCl, and 0,05% Tween®-20.
Product Family Options
Product Information
- PkAvailabilityPrice
- 786-558Church & Gilbert's [2X] buffer
- 786-559Church & Gilbert's [2X] buffer
- 786-525Denhardt solution [100X]
- 786-538TMAC Hyb Solution [3X]
- 786-470TNT Buffer
- 786-471TNT Buffer
- 786-160EKONO® hybridization buffer
- 786-528Hybridization Solution I (0.9M NaCl, 90mM Trisodium citrate, 5% Tween® 20)Hybridization Solution I (0.9M NaCl, 90mM Trisodium citrate, 5% Tween® 20)1 LLog in for availability1 * 1 L704,00 kr
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck