Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, in vitro translation, RNase protection assays and poly (A+) selection.

• Scalable and ready to use
• Immediate inhibition of RNase
• Ability to sequentially isolate RNA, DNA then protein

Tri-Xtract™ is a monophasic solution of phenol and guanidine thiocyanate that maintains the integrity of the RNA during cell disruption and homogenisation, the addition of chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The total RNA is recovered by isopropyl alcohol precipitation. The DNA and proteins can also be recovered by sequential precipitation from the organic phase.