illustra™ NICK™ columns
About this item
NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.
- For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
- Prepacked with Sephadex™ G-50 DNA Grade in distilled water with 0,15% Kathon CG/ICP Biocide
- Can accommodate sample volumes up to 100 μl
They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered.
2 Options Available Below
Product Details & Documents
NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.
- For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
- Prepacked with Sephadex™ G-50 DNA Grade in distilled water with 0,15% Kathon CG/ICP Biocide
- Can accommodate sample volumes up to 100 μl
They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered.
Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 17-0855-01
- 17-0855-02
- Description:
- Illustra™ NICK™ columns
- Illustra™ NICK™ columns
- Target molecule:
- DNA
- DNA
- Refine target molecule:
- Amplified/modified DNA
- Amplified/modified DNA
- Sample type:
- PCR and other enzymatic reactions
- PCR and other enzymatic reactions
- Method / Format:
- Gel filtration
- Gel filtration
- Prep size:
- Scalable
- Scalable
Specifications
Product Family Options
Product Information
- PkAvailabilityPrice
- 17-0855-01Illustra™ NICK™ columnsSpecifications:
More
Specifications
Cat. No.17-0855-01DescriptionIllustra™ NICK™ columnsTarget moleculeDNARefine target moleculeAmplified/modified DNASample typePCR and other enzymatic reactionsMethod / FormatGel filtrationPrep sizeScalable - 17-0855-02Illustra™ NICK™ columnsSpecifications:
More
Specifications
Cat. No.17-0855-02DescriptionIllustra™ NICK™ columnsTarget moleculeDNARefine target moleculeAmplified/modified DNASample typePCR and other enzymatic reactionsMethod / FormatGel filtrationPrep sizeScalable
Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".
Otherwise, you will receive generic recommendations.
Catalog #: 83639.320
Supplier: VWR Chemicals
Catalog #: 20864.320
Supplier: VWR Chemicals
Catalog #: 83640.320
Supplier: VWR Chemicals
Catalog #: 1.00030.2500
Supplier: Merck
Catalog #: 31770.294
Supplier: VWR Chemicals
Catalog #: 83645.320
Supplier: VWR Chemicals
Catalog #: 1.09439.1000
Supplier: Merck