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Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: Charles River Laboratories Cell Solutions

CD34 depleted mononuclear cells from mobilised peripheral blood are isolated by depleting the CD34 cells using immunomagnetic anti-CD34 microbeads.

Supplier: Charles River Laboratories Cell Solutions

CD34⁺ cells are isolated using positive immunomagnetic cell separation procedures from umbilical cord blood. Human cord blood is collected aseptically at the time of delivery from healthy pregnant women under institutional review board (IRB) approved procedures.

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

CD34⁺ stem and progenitor cells from umbilical cord blood are crucial for haematopoiesis and modulation of the immune response. These unique cells contribute to the formation of various blood cells, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M2 macrophages are derived from purified monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of M-CSF for 10 days. The M2 phenotyping was confirmed by morphology and the expression of specific biomarkers which monitored by flow cytometry. The cells were harvested after 10 days of in vitro culture.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood mononuclear cells (PBMC) are isolated from blood collected in FDA-registered collection centres, from IRB consented healthy human donors and following cGMP and cGTP collection guidelines.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

CD4+ Helper T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Rockland Immunochemicals

Whole cell lysates are derived from cell lines tissues using highly refined extraction protocols to ensure quality, protein integrity and high reproducibility. Lysates are prepared in denaturing buffer without dissociating agents.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells. These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.

Supplier: Charles River Laboratories Cell Solutions

Bone marrow is a major source of stem cells, which are being used in cancer research, cell studies, transplantation studies, and other research.

Supplier: PROVITRO

Natural Killer cells (NK cells) constitute a lymphocytic subset of the immune system and play a crucial role in immunological surveillance and host defence. Their significance spans across various areas such as bone marrow transplantation, cancer, pregnancy, autoimmune diseases, viral infections, and increasingly in adaptive immunity.

Supplier: Charles River Laboratories Cell Solutions

Naive T cells are isolated by depleting CD45RO^₊ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naive T cells are phenotypically classified as CD45⁺, CD^₊, and CD45RA^₊.

Supplier: Charles River Laboratories Cell Solutions

Human mobilised peripheral blood CD133⁺ stem/progenitor cells are isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: PROVITRO

B cells have been isolated from mononuclear cells through either positive or negative selection using immunomagnetic cell separation techniques.

Supplier: Charles River Laboratories Cell Solutions

CD3⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

Human peripheral blood mononuclear cells (PBMCs) are utilised as source material for isolating lymphocytes (T cells, B cells and NK cells) and monocytes in research and across fields such as immunology, immuno-oncology, and cell and gene therapies.
All PBMCs are isolated through Ficoll density gradient separation from freshly obtained leukapheresis ACD-A packs and directly cryopreserved.

Supplier: Charles River Laboratories Cell Solutions

CD8⁺ cytotoxic T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

Dental Pulp Stem Cells (DPSCs) are primarily sourced from the pulp tissues of deciduous teeth, primary incisors, and permanent third molars. The dental pulp, especially from third molars, is noted for its rich reservoir of stem cells. DPSCs derived from third molars express key pluripotency-associated transcription factors including oct4, sox2, klf4, nanog, and c-myc.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: ENZO LIFE SCIENCES

The PC-12 cell line was derived from a transplantable rat adrenal pheochromocytoma. These cells were maintained in Ham's F12K medium with L-glutamine (2 mM) adjusted to contain 1,5 g/L sodium bicarbonate (82,5%), horse serum (15%) and faetal bovine serum (2,5%).

Supplier: Charles River Laboratories Cell Solutions

CD56⁺ NK cells are isolated from mononuclear cells either by positive selection using an antibody that targets the CD56 antigen, or by negative selection using immunomagnetic cell separation procedures.

Supplier: PROVITRO

Human Adipose-Derived Stem Cells (ADSCs) are extracted from human lipoaspirate tissue obtained during elective liposuction surgeries. These cells are available from both healthy donors and patients. ADSCs exhibit phenotypic and functional characteristics similar to bone marrow-derived mesenchymal stem cells.

Supplier: Charles River Laboratories Cell Solutions

Human mononuclear cells are isolated from IRB-consented volunteer donors diagnosed with HIV or AIDS.

Supplier: Charles River Laboratories Cell Solutions

The mononuclear cell is a widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M1 macrophages are derived from purified monocytes. CD14⁺ monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures and were cultured in the presence of GM-CSF for 10 days. The M1 phenotyping was confirmed by morphology and the expression of specific biomarkers.