You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Nucleic acid and protein destain, Silver D-Stain™
Supplier: G-Biosciences
The staining of gels with silver stains often results in over-staining or unevenly stained gels. Silver D-Stain™ eliminates the need to repeat the electrophoresis.
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Restore™ Western Blot Stripping Buffer
Supplier: Thermo Fisher Scientific
Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.
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Sample clean up, Perfect-FOCUS™
Supplier: G-Biosciences
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
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Rehydration reagent, DeStreak
Supplier: Cytiva
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
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Peroxidase Indicator Reagent
Supplier: SIGMA ALDRICH MICROSCOPY
It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.
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VWR®, Destaining bags
Supplier: VWR Chemicals
Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.
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Ethidium bromide adsorber for decontamination of ethidium bromide from staining solutions, Sigma-Aldrich®
Supplier: Merck
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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Western blot assay kits, ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.
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Chemiluminescent acridinium esters
Supplier: ENZO LIFE SCIENCES
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.
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Eye tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.
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Human cancer cell line blot
Supplier: G-Biosciences
Ready to screen human cancer cell line blots.
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Chemicon® ReBlot Plus Kits
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities
Supplier: Merck
Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.
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Nitrotyrosine BSA for immunoblotting experiments
Supplier: Cayman Chemical
This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.
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Western blots, pre-made, mouse subcellular fraction specific blot
Supplier: G-Biosciences
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
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picoLUCENT™ PLUS-HRP Kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Immunodetection reagents, femtoLUCENT™ PLUS
Supplier: G-Biosciences
The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.
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Immobilon™ Block Noise Canceling Reagents
Supplier: Merck
Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.
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Immunoblotting kits, MaxTag™
Supplier: Rockland Immunochemicals
These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.
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Cell extract HL60 (non-induced)
Supplier: ENZO LIFE SCIENCES
Whole-cell extract of human HL-60 leukemia cells.
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Kidney tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.
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picoLUCENT™ PLUS-AP kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Chemiluminescent detection reagents, Immobilon® Western HRP substrates
Supplier: Merck
Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.
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Single tissue blots, multiple species
Supplier: G-Biosciences
Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.
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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X
Supplier: Merck
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
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Chemiluminescent detection reagents, Luminata™ Western HRP substrates
Supplier: Merck
Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.