You searched for: Electrophoresis Reagents

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

Supplier: VWR Chemicals

Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.

Supplier: Merck

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

Supplier: G-Biosciences

The staining of gels with silver stains often results in over-staining or unevenly stained gels. Silver D-Stain™ eliminates the need to repeat the electrophoresis.

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

Supplier: VWR Chemicals

VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.

Supplier: Molecular Devices

The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

Supplier: G-Biosciences

These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

Supplier: G-Biosciences

Ready to screen human cancer cell line blots.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: Merck

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Merck

Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

Supplier: Merck

Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.