You searched for: Nucleic Acid Reagents

Supplier: Cytiva

Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.

Supplier: MP Biomedicals

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

Supplier: Corning

The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

Supplier: Cytiva

Sera-Xtracta genomic DNA kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.

Supplier: MP Biomedicals

SPINeasy PCR purification and gel extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.

Supplier: Cytiva

The ExoProStar™ S PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. illustra ExoProStar™ S improves digestion efficiency with no degradation of the target PCR product. Sample remain completely intact and ready for immediate use in manual or automated processes. For maximum flexibility the enzymes are supplied in two separate tubes.

Supplier: MP Biomedicals

SPINeasy Virus RNA kit is a silica-membrane spin-column kit that enables quick and convenient extraction of virus RNA from cell culture media and bodily fluids such as saliva and serum, without the use of toxic substances such as phenol and chloroform. Through a simple workflow, virus RNA is typically extracted within 20 minutes and is immediately available for downstream applications such as RT-PCR and RT-qPCR.

Supplier: Cytiva

NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.

Supplier: G-Biosciences

The majority of genomic DNA extraction methods involve numerous physical manipulations, including mixing, pipetting, shaking, binding to resin, elution, which results in sheared DNA that may not be suitable for further analysis.

Supplier: MP Biomedicals

MagBeads Fast Circulating DNA Kit is intended for purification of high-quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Supplier: MP Biomedicals

Host DNA contamination impedes the molecular analyses of microbiomes in host samples, such as bodily fluids and swabs. The SPINeasy Host Depletion Microbial DNA kit provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA.

Supplier: MP Biomedicals

The MagBeads FastDNA Kit for Feces allows thorough sample lysis and quick isolation of DNA from fecal samples. It is using magnetic beads with high DNA binding capacity for extraction of high yields of pure DNA. This is done manually using a magnetic rack or adapted to automated instruments for high throughput processing.

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Supplier: Cytiva

SeraSil-Mag™ silica coated superparamagnetic beads for nucleic acid isolation deliver high purity DNA extraction in NGS applications. These beads provide an optimal surface for nucleic acid binding with high performance and low background.

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

Supplier: Genscript

The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from Escherichia coli cultures.

Supplier: MP Biomedicals

Soil samples are complex environments characterised by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components which may prove to be challenging for downstream analyses.

Supplier: MP Biomedicals

The SPINeasy® DNA Pro Kit for Feces is able to handle a wide range of fecal samples.

Supplier: MP Biomedicals

Quick and convenient purification of total RNA from animal tissues, plant tissues and tissue cultures using spin column technology.

Supplier: MP Biomedicals

SPINeasy DNA Kit for plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed.

Supplier: MP Biomedicals

MagBeads FastRNA Kit for FFPE is intended for rapid extraction of RNA from tissue, cells, blood, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Supplier: MP Biomedicals

The GeneClean® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with this kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

Supplier: Cytiva

The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

Supplier: PRIMERDESIGN

The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.

It is fast, and incredibly easy to perform.

Supplier: AAT BIOQUEST

MP650 is designed to have identical spectra to those of Mustang Purple.

Supplier: AAT BIOQUEST

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.

Supplier: AAT BIOQUEST

FAM dye qPCR calibration solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.

Supplier: FINNZYMES REAGENTS

Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.