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Nucleic Acid Reagents

Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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Restriction enzymes, NotI, 50 U/µl, Fermentas

Restriction enzymes, NotI, 50 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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AccuStart™ master mixes, long range SuperMix

AccuStart™ master mixes, long range SuperMix

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

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T7 RNA polymerase

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

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Mag-Bind® Plant DNA DS 96 kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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DNA Library Prep Kit, sparQ

DNA Library Prep Kit, sparQ

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

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DNA Concentrator Kit, Abcam

Supplier: Abcam

The DNA Concentrator Kit (ab156895) is a complete set of essential components which enables the experimenter to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin-embedded tissues.

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Mitochondria/Cytosol Fractionation Kit, Abcam

Supplier: Abcam

Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays

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Montage® SEQ96 Sequencing Reaction Cleanup Kits

Montage® SEQ96 Sequencing Reaction Cleanup Kits

Supplier: Merck

The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.

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Viral RNA Xpress kit, Mag-Bind®

Viral RNA Xpress kit, Mag-Bind®

Supplier: OMEGA BIO-TEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.

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TriLink® Engineered RNase Inhibitor

TriLink® Engineered RNase Inhibitor

Supplier: TriLink BioTechnologies

Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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TriLink® Uridine-5'-Triphosphate

TriLink® Uridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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AccuStart™ II PCR ToughMix®

AccuStart™ II PCR ToughMix®

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

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E.Z.N.A.® Endo-Free plasmid kits

E.Z.N.A.® Endo-Free plasmid kits

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

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E.Z.N.A.® Plant & Fungal DNA Kit

E.Z.N.A.® Plant & Fungal DNA Kit

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

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