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Blotting Apparatus

VWR offers a variety of different blotting apparatus for Southern, Western, and Northern dot blotting procedures. Electroblotting systems feature easy blotting of multiple gels simultaneously in less than an hour. Mini and large tank systems produce uniform and consistent results. Semi-dry blotting systems offer fast and efficient transfers of proteins to membranes with low buffer volume. Most blotting apparatus include essential accessories.

 
VWR® shiroGEL Mini PAGE Electroblotting System

VWR® shiroGEL Mini PAGE Electroblotting System

Supplier: VWR Collection

The shiroGEL Mini PAGE electroblotting system allows quick and easy blotting of three gels simultaneously. Gels fit snugly in blotting cassettes with transfer membranes, which are then placed into the blotting insert. Coiled electrodes within the insert generate a high intensity current, allowing standard transfer to take place in approximately one hour.

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VWR® PerfectBlue, Tank Blotters

VWR® PerfectBlue, Tank Blotters

Supplier: VWR Collection

PerfectBlue S and M tank electroblotters are designed to rapidly transfer nucleic acid or protein fragments from up to four polyacrylamide gels at a time to nitrocellulose, nylon or PVDF membranes. The colour coded cassettes allow for easy assembly of transfer sandwich and error-free transfer. The large buffer capacity and integral cooling allow extended transfer times and high voltage/high current operation for the transfer of high molecular weight proteins.

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VWR® PerfectBlue, Semi-dry electroblotters

VWR® PerfectBlue, Semi-dry electroblotters

Supplier: VWR Collection

PerfectBlue semi-dry electroblotters are designed for clean, rapid and effective transfer of proteins and nucleic acids from polyacrylamide or agarose gels to membranes. Suitable for use with Western, Southern and Northern blotting, these semi-dry blotters reduce many lengthy procedures down to around an hour.

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Blot module, miniVE™

Supplier: HOEFER ELECTROPHORESIS

Blotting apparatus, Blot module, miniVE™

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VWR® kuroGEL, Semi-dry blotter

VWR® kuroGEL, Semi-dry blotter

Supplier: VWR Collection

The kuroGEL semi-dry blotter provides great flexibility in a laboratory since it can be used for all types of blotting: Western, Southern and Northern. Blotting is fast and easy, and can typically be completed in 15 to 30 min. Set-up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes, while the platinum coated electrodes provide uniform pressure ensuring even transfers.

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Semi-dry transfer unit, TE77X

Semi-dry transfer unit, TE77X

Supplier: HOEFER ELECTROPHORESIS

Semi-dry transfer unit, TE77X

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Dot blot system, Enhancer™

Dot blot system, Enhancer™

Supplier: G-Biosciences

Enhancer™ dot blot system allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12×9 cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion.

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Semi-dry electroblotters, Owl™ HEP-1 and HEP-3

Semi-dry electroblotters, Owl™ HEP-1 and HEP-3

Supplier: THERMO OWL SCIENTIFIC

These sleek, low profile systems are designed for efficient transfer of proteins and nucleic acids to membranes. The base contains a stainless steel cathode plate and the lid houses a platinum/titanium anode plate.

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Electro-blotting systems, mini and large tanks, Owl™

Electro-blotting systems, mini and large tanks, Owl™

Supplier: THERMO OWL SCIENTIFIC

Blotting apparatus, Owl™, VEP-3 mini tank electro-blotting system, including transfer area: 200×185 mm, buffer volume: 4 l, L×W×H: 90×300×300 mm

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Western blot roller, Pierce™

Western blot roller, Pierce™

Supplier: Thermo Fisher Scientific

The Western blot roller is a simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.

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Whatman™ TurboBlotter Kits and Refills, Whatman products (Cytiva)

Whatman™ TurboBlotter Kits and Refills, Whatman products (Cytiva)

Supplier: Whatman products (Cytiva)

The TurboBlotter™ system is a rapid, downward blotting device for high-resolution transfer of DNA and RNA. The conventional Northern/Southern transfer stack has been turned upside down in order to take advantage of gravity.

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Blot module, miniVE™

Blot module, miniVE™

Supplier: Cytiva

Perform 1-D and 2-D electrophoresis and electrotransfer in a single compact unit.

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Fast wet transfer system for mini gels, eBlot® L1

Fast wet transfer system for mini gels, eBlot® L1

Supplier: Genscript

eBlot® L1 wet protein transfer system is a highly efficient western blotting transfer system that uses a novel technology (patent pending) developed by GenScript. eBlot® L1 combines the high reproducibility and efficiency of the traditional wet transfer with the speed and convenience of other fast transfer systems. The device allows efficient blot transfer of small, medium and large molecular weight proteins within 9 to 17 minutes.

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Transfer tanks, Transphor™

Transfer tanks, Transphor™

Supplier: Cytiva

Transphor™ units use transverse electrophoresis to transfer proteins from polyacrylamide gels onto nitrocellulose and PVDF membranes. The optional Transphor™ power lid delivers up to 1,5 A and 100 V in constant current mode.

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Vacuum blotter, TransDNA Express™, MP Biomedicals

Supplier: MP Biomedicals

TransDNA Express™ vacuum blotter is used to quickly and efficiently transfer DNA and RNA to membranes. Transfer is completed within an hour without any additional handling.

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Automated western device, eZwest™ Lite

Automated western device, eZwest™ Lite

Supplier: Genscript

eZwest™ Lite is an automated western blotting device providing an easy and simple solution in your western blotting workflow. After proteins are transferred onto the membrane, load the membrane into our eZwest™ Lite device for automated blocking, incubation, and washing cycles. The user only needs to prepare the required reagents, run the customised program, and eZwest™ Lite will take care of the rest. Once the program is finished, the user can collect the results and immediately proceed to detection and analysis steps. eZwest™ Lite is easy to use and has minimal footprint.

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