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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Colorimetric NAD/NADH Ratio Assay Kit

Amplite® Colorimetric NAD/NADH Ratio Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Yeast β-galactosidase assay kit, Pierce™

Yeast β-galactosidase assay kit, Pierce™

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

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Amplite® Fluorimetric Melanin Assay Kit

Amplite® Fluorimetric Melanin Assay Kit

Supplier: AAT BIOQUEST

Melanins have very diverse roles and functions in various organisms.

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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.

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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

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Amplite® Luminometric Nitroreductase Assay Kit

Amplite® Luminometric Nitroreductase Assay Kit

Supplier: AAT BIOQUEST

Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.

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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit

Supplier: AAT BIOQUEST

Site-selective modification of proteins is of major interest in chemical biology.

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Amplite® Colorimetric D-Lactate Assay Kit

Amplite® Colorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.

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Amplite® Fluorimetric Mercuric Ion Quantitation Kit

Amplite® Fluorimetric Mercuric Ion Quantitation Kit

Supplier: AAT BIOQUEST

Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.

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Amplite® Choline Quantitation Kit

Amplite® Choline Quantitation Kit

Supplier: AAT BIOQUEST

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).

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Amplite® Fluorimetric Ascorbic Acid Assay Kit

Amplite® Fluorimetric Ascorbic Acid Assay Kit

Supplier: AAT BIOQUEST

L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.

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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: AAT BIOQUEST

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.

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Quantitative peroxide assay kit (aqueous), Pierce™

Quantitative peroxide assay kit (aqueous), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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ReadiUse™ NADP Regenerating Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.

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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*

Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*

Supplier: AAT BIOQUEST

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Phosphatase assay kit

Phosphatase assay kit

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

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Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules.

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Amplite® Colorimetric Aldehyde Quantitation Kit

Amplite® Colorimetric Aldehyde Quantitation Kit

Supplier: AAT BIOQUEST

Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance.

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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit is an essential tool for researchers involved in bioconjugation research, specifically designed for the accurate quantification of maleimide functional groups on solid surfaces such as magnetic beads, polystyrene beads, or other microsphere nanoparticles.

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Amplite® Blue Peroxidase Substrate

Amplite® Blue Peroxidase Substrate

Supplier: AAT BIOQUEST

A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.

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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*

Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*

Supplier: AAT BIOQUEST

Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.

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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*

Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Colorimetric Acetylcholinesterase Assay Kit

Amplite® Colorimetric Acetylcholinesterase Assay Kit

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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