Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT BIOQUEST
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT BIOQUEST
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Fluorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.
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Renilla luciferase flash assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
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Amplite® Colorimetric Total NADP and NADPH Assay Kit
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Cypridina luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Quantitative peroxide assay kit (lipid), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.
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Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT BIOQUEST
Malondialdehyde (MDA) is natural byproduct of lipid peroxidation and is widely used as a key indicator to determine the oxidative stress and free radical formation.
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Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated APC proteins using a proprietary sensor, Maleimide 555™, which has an absorption peak at ~555 nm.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit
Supplier: AAT BIOQUEST
E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT BIOQUEST
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric L-Lactate Assay Kit
Supplier: AAT BIOQUEST
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
Supplier: AAT BIOQUEST
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Colorimetric Lipase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.