Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Myeloperoxidase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
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Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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NUCLEOLAR-ID® Green detection kit
Supplier: ENZO LIFE SCIENCES
Specifically designed for visualizing microscopically nucleoli in living cells.
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QBT™ Fatty acid uptake assay kit
Supplier: Molecular Devices
QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.
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CREB (Phospho-Ser133) transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of CREB.
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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit
Supplier: ENZO LIFE SCIENCES
The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.
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Green detection kit, LYSO-ID®
Supplier: ENZO LIFE SCIENCES
LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Prostaglandin Screening Library II
Supplier: Cayman Chemical
For D and E series prostaglandin analog screening.
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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Autophagy detection tests, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.
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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.
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NUCLEAR-ID® green chromatin condensation detection kit
Supplier: ENZO LIFE SCIENCES
This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.
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DPPIV/CD26 assay kit for biological samples
Supplier: ENZO LIFE SCIENCES
The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.
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ViaFluor® 405 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with blue fluorescence.
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Potassium assay kits, FLIPR®
Supplier: Molecular Devices
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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Hypoxia/oxidative stress detection kit, ROS-ID®
Supplier: ENZO LIFE SCIENCES
The ROS-ID® Hypoxia/Oxidative Stress Detection Kit is a specific detection system designed for functional detection of hypoxia and oxidative stress levels in live cells (both suspension and adherent) using fluorescent microscopy or flow cytometry.
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Calcein-AM cell viability assay kits
Supplier: Biotium
Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.
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PMA™, PMAxx™, Real-Time PCR Viability Kit
Supplier: Biotium
Viability PCR With PMA and PMAxx™ dyes
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Neurotransmitter transporter uptake assay kits
Supplier: Molecular Devices
The Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of dopamine, norepinephrine and serotonin transporter (SERT, NET and DAT, respectively) activity in expressing cells.
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CF® Dye Dextran 70000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.
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CF® Dye Dextran 250000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.
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CF® Dye Dextran 10000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.
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Membrane potential detection kit, MITO-ID®
Supplier: ENZO LIFE SCIENCES
MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.
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Fatty Acid Screening Library
Supplier: Cayman Chemical
For screening of variety of fatty acids.
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LysoView™ Lysosomal Stain
Supplier: Biotium
LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.
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ROS-ID™ total ROS detection kit
Supplier: ENZO LIFE SCIENCES
This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.
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Aggresome detection kit, PROTEOSTAT®
Supplier: ENZO LIFE SCIENCES
PROTEOSTAT® Aggresome Detection Kit is a robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology.
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Green detection kit, MITO-ID®
Supplier: ENZO LIFE SCIENCES
MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.
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Mitochondria/cytosol fractionation kit
Supplier: ENZO LIFE SCIENCES
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.