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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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PUR-1

PUR-1

Supplier: AAT Bioquest

PUR-1 is essentially non-fluorescent in the absence of nucleic acids.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: Molecular Devices

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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CytoScan™ Cell proliferation assay kit, WST-1

CytoScan™ Cell proliferation assay kit, WST-1

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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SEAP assay kit

SEAP assay kit

Supplier: OZ BIOSCIENCES

SEAP assay kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues.

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CytoTell™ Orange

CytoTell™ Orange

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyse heterogeneous cell populations.

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Senescence kit

Senescence kit

Supplier: OZ BIOSCIENCES

Senescence kit allows visualisation of aging cells and is ideal when cells in culture show abnormalities typical of the Hayflick limit after a long period of normal growth. The cells vary in size and shape, the cytoplasm begins to be granular with many cell inclusions and debris formed in the medium.

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CytoScan™-fluoro cytotoxicity assay kit

CytoScan™-fluoro cytotoxicity assay kit

Supplier: G-Biosciences

The CytoScan™-fluoro Cytotoxicity assay is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluorescent compound (resazurin) to a fluorescent compound (resorufin), which is measured using a fluorometer. The assay detects even low level damage to cell membrane not detected with other methods.

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FunXite™-1

FunXite™-1

Supplier: AAT Bioquest

FunXite™-1 is chemically same molecule to FUN® 1 (FUN® 1 is the trademark of ThermoFisher).

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DiTO™-3  [equivalent to TOTO®-3] *5 mM DMSO Solution* (0.2 ml)

DiTO™-3 [equivalent to TOTO®-3] *5 mM DMSO Solution* (0.2 ml)

Supplier: AAT Bioquest

DiTO™-3 is chemically equivalent to TOTO®-3 (TOTO® is the trademark of Invitrogen).

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CellPaint™ TSP membrane stain

CellPaint™ TSP membrane stain

Supplier: AAT Bioquest

TSP is a styrylpyridine-based fluorescent membrane probe suitable for imaging plasma membranes in living cells and tissues.

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DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* (0.2 ml)

DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* (0.2 ml)

Supplier: AAT Bioquest

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen).

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LysoBrite™ Red

LysoBrite™ Red

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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CYTO-13

CYTO-13

Supplier: AAT Bioquest

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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DiYO™-1 [Equivalent To YOYO®-1]

DiYO™-1 [Equivalent To YOYO®-1]

Supplier: AAT Bioquest

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen).

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LysoBrite™ Blue

LysoBrite™ Blue

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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MitoLite™ Green EX488

MitoLite™ Green EX488

Supplier: AAT Bioquest

MitoLite™ Green is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient.

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LRB Red™ Succinimidyl ester

LRB Red™ Succinimidyl ester

Supplier: AAT Bioquest

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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LysoBrite™ Green

LysoBrite™ Green

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Expand 1 Items
 
CytoTell™ UltraGreen

CytoTell™ UltraGreen

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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CytoTell™ Green

CytoTell™ Green

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyse heterogeneous cell populations.

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Cell viability assay kits, OZBlue

Cell viability assay kits, OZBlue

Supplier: OZ BIOSCIENCES

OZBlue cell viability kit is a ready to use assay system based on the fluorimetric/colorimetric quantification of metabolic activity in living cells. Resazurin dye is used as an indicator of cell viability.

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MESG *Phosphate Assay Reagent*

MESG *Phosphate Assay Reagent*

Supplier: AAT Bioquest

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Potassium assay kits, FLIPR®

Potassium assay kits, FLIPR®

Supplier: Molecular Devices

The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.

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