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You searched for: Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

CF® Dye Dextran 250000 MW, anionic and fixable

CF® Dye Dextran 250000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

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Neurotransmitter transporter uptake assay kits

Neurotransmitter transporter uptake assay kits

Supplier: Molecular Devices

The Neurotransmitter Transporter Uptake Assay Kit provides a fast, simple and reliable fluorescence-based assay for the detection of dopamine, norepinephrine and serotonin transporter (SERT, NET and DAT, respectively) activity in expressing cells.

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Bacterial viability and gram stain kit

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

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Membrane potential detection kit, MITO-ID®

Membrane potential detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

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Green long-term cell tracer, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

Supplier: Biotium

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

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Hydrogen peroxide fluorometric detection kit

Supplier: ENZO LIFE SCIENCES

The Hydrogen Peroxide Fluorometric Detection Kit provides fast results in just 10 minutes for the measurement of either hydrogen peroxide or peroxidase activity in a convenient one-step homogeneous assay.

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Apoptosis assay kit, NucView® 488 and MitoView™ 633

Apoptosis assay kit, NucView® 488 and MitoView™ 633

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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Flow cytometry fixation/permeabilisation kit

Flow cytometry fixation/permeabilisation kit

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

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Calcium assay kit, FluoForte®

Calcium assay kit, FluoForte®

Supplier: ENZO LIFE SCIENCES

FluoForte® Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence.

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Apoptosis inducer set

Supplier: ENZO LIFE SCIENCES

The set contains five reagents that induce apoptosis through different mechanisms.

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Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Apoptosis/Necrosis Detection Kit is a multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes.

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NETosis Assay Kit

NETosis Assay Kit

Supplier: Cayman Chemical

For the measurement of NET-derived neutrophil elastase.

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PPARδ transcription factor assay kit

PPARδ transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

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Mitochondria/cytosol fractionation kit

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

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S-Glutathionylated Protein Detection Kit

S-Glutathionylated Protein Detection Kit

Supplier: Cayman Chemical

A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.

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Apoptosis and necrosis quantitation kits

Apoptosis and necrosis quantitation kits

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

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ROS-ID™ total ROS/superoxide detection kit

ROS-ID™ total ROS/superoxide detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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Glutathione Peroxidase Assay Kit

Glutathione Peroxidase Assay Kit

Supplier: Cayman Chemical

For the measurement of glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

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ROS-ID™ NO Detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

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Red long-term cell tracer, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID® is a live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

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Annexin V-FITC apoptosis detection kit

Supplier: ENZO LIFE SCIENCES

For the detection of apoptotic cells by flow cytometry.

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Annexin V-EGFP apoptosis detection kit

Supplier: ENZO LIFE SCIENCES

For the detection of apoptotic cells by flow cytometry.

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sPLA2 activity kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.

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Annexin V CF® dye conjugates, azide-free, lyophilised

Supplier: Biotium

Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.

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MaxSpec® Dienes Derivatization Kit

MaxSpec® Dienes Derivatization Kit

Supplier: Cayman Chemical

Derivatization Kit for the sensitive LC-MS/MS analysis of dienes such as Vitamin D and Vitamin D metabolites.

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Green detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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