You searched for: Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
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Cell-based assay calcein AM
Supplier: Cayman Chemical
Cellular assay, Cell-based assay calcein AM
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Glucose (HK) assay kit
Supplier: Merck
The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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Phagocytosis assay kit (IgG FITC)
Supplier: Cayman Chemical
Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Triglyceride colorimetric assay kit
Supplier: Cayman Chemical
Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.
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Cytoplasmic membrane labelling kits, CellBrite™
Supplier: Biotium
CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.
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Millicell® µ-Migration Assay Kit
Supplier: Merck
This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.
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ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit
Supplier: Molecular Devices
SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.
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Fluorescent nerve terminal probes and stains
Supplier: Biotium
Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.
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ViaFluor® 647 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with far-red fluorescence.
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Thiol Detection Assay Kits
Supplier: Cayman Chemical
A simple, reproducible, and sensitive tool for assaying free thiol content in samples.
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CellBrite™ NIR cytoplasmic membrane dyes
Supplier: Biotium
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are near-infrared lipophilic carbocyanine dyes for labeling cytoplasmic membranes in live or formaldehyde-fixed cells.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Big blue
Supplier: AGILENT
These are used in all Big Blue rodents and cell lines.
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Nitric oxide reagents and kits
Supplier: Biotium
Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.
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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium
Supplier: Biotium
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
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Firefly luciferase assay kit (Lyophilised)
Supplier: Biotium
This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.
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Cell Meter™ Fluorimetric Cellular Voltage Assay Kit
Supplier: AAT BIOQUEST
Almost all plasma membranes have an electrical potential across them, with the inside usually negative with respect to the outside.
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Neutrophil/Monocyte Respiratory Burst Assay Kit
Supplier: Cayman Chemical
Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
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LDH Cytotoxicity Assay Kit
Supplier: Cayman Chemical
For measurement of LDH activity as an indicator of cell death.
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NF[kappa]B transcription factor assay kits
Supplier: Rockland Immunochemicals
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
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TUNEL assay kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.
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Uric acid assay kit
Supplier: Cayman Chemical
Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.
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Cell proliferation assay kit, WST-1
Supplier: Cayman Chemical
WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.
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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium
Supplier: Biotium
RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.
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S-Glutathionylated Protein Detection Kit
Supplier: Cayman Chemical
A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.