Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Cell integrity kit, EarlyTox™
Supplier: Molecular Devices
The EarlyTox™ Cell Integrity Kit enables the differentiation of live cells from dead cells via fluorescent labeling. This is useful for the rapid quantification of cell viability when used with the SpectraMax® MiniMax™ 300 Imaging Cytometer or other fluorescence-based cellular imaging instruments.
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Foxp3/transcription factor staining buffer
Supplier: Tonbo Biosciences
Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.
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CREB (Phospho-Ser133) transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of CREB.
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Prostaglandin Screening Library II
Supplier: Cayman Chemical
For D and E series prostaglandin analog screening.
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Fatty Acid Screening Library
Supplier: Cayman Chemical
For screening of variety of fatty acids.
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NF[kappa]B transcription factor assay kits
Supplier: Rockland Immunochemicals
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
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LipidLaunch™ LNP-0315 Uptake Kit (Green Fluorescence)
Supplier: Cayman Chemical
Cayman’s LipidLaunch™ LNP-0315 uptake kit (Green Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs by their target cell type without the need for specialised equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.
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PKC Pathway Modulator Set Abcam
Supplier: Abcam
PKC Pathway Modulator Set (ab284176) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Wnt/b-Catenin Pathway Modulator Set Abcam
Supplier: Abcam
Wnt/b-Catenin Pathway Modulator Set (ab284178) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Cell Proliferation Kit XTT (1000 tests)
Supplier: Apollo Scientific
Cell Proliferation Kit XTT (1000 tests)
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Cell-based assay calcein AM
Supplier: Cayman Chemical
Cellular assay, Cell-based assay calcein AM
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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium
Supplier: Biotium
JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.
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CellBrite™ NIR cytoplasmic membrane dyes
Supplier: Biotium
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are near-infrared lipophilic carbocyanine dyes for labeling cytoplasmic membranes in live or formaldehyde-fixed cells.
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Calcein-AM cell viability assay kits
Supplier: Biotium
Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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NETosis Assay Kit
Supplier: Cayman Chemical
For the measurement of NET-derived neutrophil elastase.
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