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You searched for: Stains

Stains

Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.

VWR® Cryolab Q Path, Freezing Aerosol

VWR® Cryolab Q Path, Freezing Aerosol

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

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Glycoprotein stain

Glycoprotein stain

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

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VWR® Q Path® Safemount, Mounting Agents for Microscopy

VWR® Q Path® Safemount, Mounting Agents for Microscopy

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

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Epoxy Embedding Medium Kit

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

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VWR® Q Path®, Medium-acting decalcifiers

VWR® Q Path®, Medium-acting decalcifiers

Supplier: VWR Chemicals

This medium-acting decalcifier contains a mixture of hydrochloric acids.

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VWR® Q Path®, Slow Acting Decalcifiers

VWR® Q Path®, Slow Acting Decalcifiers

Supplier: VWR Chemicals

DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.

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VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade

VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade

Supplier: VWR Chemicals

Decalcifier according to Kristenson.

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VWR® HistoChoice®, Clearing agent

VWR® HistoChoice®, Clearing agent

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

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VWR® Fixing Solution

VWR® Fixing Solution

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

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J.T.Baker® Ultrakitt, Mounting Medium, Histo Grade

Supplier: Avantor

Mounting medium for coverslipping of slides.

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VWR® Q Path® Labofix Cytology Fixative

VWR® Q Path® Labofix Cytology Fixative

Supplier: VWR Chemicals

Q Path Labofix is a spray cytology fixative able to fix and store the cellular elements from gynaecological and non gynaecological smears after spreading them out on a glass slide.

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VWR® Q Path® DC3, Decalcifier Rapid-Acting

VWR® Q Path® DC3, Decalcifier Rapid-Acting

Supplier: VWR Chemicals

Contains a mixture of hydrochloric acid and alcohols with some EDTA.

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VWR® Mollifex™, Softening agent

VWR® Mollifex™, Softening agent

Supplier: VWR Chemicals

Mollifex™ is used to soak the cut face of paraffin embedded tissue blocks prior to sectioning is useful to facilitate the mechanical process of sectioning.

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VWR® Q Path® Isomount 2000, Mounting Media

VWR® Q Path® Isomount 2000, Mounting Media

Supplier: VWR Chemicals

Mounting media based on isopropyl alcohol. Compatible with most solvent replacing agents. Suitable for manual and automatic mounting of slides. PET bottle of 500 ml.

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VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy

VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

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CytoLiner™ Fixed Cell Membrane Stains

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

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Silver stain, FOCUS™ FASTsilver™

Silver stain, FOCUS™ FASTsilver™

Supplier: G-Biosciences

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

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CYGREEN® Nucleic acid Dye

Supplier: ENZO LIFE SCIENCES

Nucleic acid dye for gel staining and higher sensitivity qPCR.

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VWR® Q Path® Cytofix, Cytology Fixture

VWR® Q Path® Cytofix, Cytology Fixture

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

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Nucleic acid stains, PAGE GelRed™

Nucleic acid stains, PAGE GelRed™

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

    
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Water-free mounting medium, Neo-Mount®

Water-free mounting medium, Neo-Mount®

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

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DNAzure® Blue Nucleic acid gel stain, 100X, Biotium

Supplier: Biotium

DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.

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BLOT-FastStain™

BLOT-FastStain™

Supplier: G-Biosciences

BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

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GelRed® Prestain Plus 6X DNA loading dye

GelRed® Prestain Plus 6X DNA loading dye

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

    
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LabSafe™ GEL blue for staining of polyacrylamide gels

Supplier: G-Biosciences

LabSafe GEL Blue™ is an enhanced protein stain based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualisation.

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GelRed® & GelGreen® Nucleic acid gel stains

GelRed® & GelGreen® Nucleic acid gel stains

Supplier: Biotium

GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.

    
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VWR® Q Path® Coverquick, Mounting Agents for Microscopy

VWR® Q Path® Coverquick, Mounting Agents for Microscopy

Supplier: VWR Chemicals

A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).

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IHC mount, HIGHDEF®

Supplier: ENZO LIFE SCIENCES

IHC mount is an aqueous-based permanent, high stability mounting medium designed for use with chromogenic labeling. Use for mounting of tissue specimens stained with peroxidase and alkaline phosphatase-based systems and various fluorescent dyes.

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Antifade mounting medium

Supplier: ENZO LIFE SCIENCES

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

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Gel loading dye, orange (6X)

Gel loading dye, orange (6X)

Supplier: Thermo Fisher Scientific

Oange loading dye (6X) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Ideal for conventional DNA electrophoresis.

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