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339 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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PSA™ iFluor® 555 Imaging Kit with Goat Anti-Rabbit IgG

PSA™ iFluor® 555 Imaging Kit with Goat Anti-Rabbit IgG

Supplier: AAT BIOQUEST

iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG

PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG

Supplier: AAT BIOQUEST

iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

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Pierce™ ABC Staining Kits

Pierce™ ABC Staining Kits

Supplier: Thermo Fisher Scientific

ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

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Amine reactive biotinylation kits, EZ-Link™

Amine reactive biotinylation kits, EZ-Link™

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit "Microscale Optimised for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit "Microscale Optimised for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.

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5-VIC Phosphoramidite

5-VIC Phosphoramidite

Supplier: AAT BIOQUEST

VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kits (2 Labelings)

Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labelling Kits (2 Labelings)

Supplier: AAT BIOQUEST

APC-Cy5.5 is a popular colour used in flow cytometry.

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ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology* (3 Reactions)

ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology* (3 Reactions)

Supplier: AAT BIOQUEST

Biotin is widely used for labeling biomolecules, in particular, antibodies.

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ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid iFluor® 350 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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Desthiobiotinylation and pull-down kit, EZ-Link™

Desthiobiotinylation and pull-down kit, EZ-Link™

Supplier: Thermo Fisher Scientific

EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

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ReadiLink™ Cy3 Oligo and ssDNA Labelling Kit

ReadiLink™ Cy3 Oligo and ssDNA Labelling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Buccutite™ MTA-Dye 650  (2 µmoles)

Buccutite™ MTA-Dye 650 (2 µmoles)

Supplier: AAT BIOQUEST

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

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ReadiLink™ Rapid Cy5 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid Cy5 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

Cy5 is one of the most popular fluorescent labelling dyes for preparing orange-red fluorescent bioconjugates.

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ReadiLink™ Rapid XFD750 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

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Helixyte™ iFluor® 555 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 555 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 555 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 350 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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ReadiLink™ KLH Conjugation Kit (2 Reactions)

ReadiLink™ KLH Conjugation Kit (2 Reactions)

Supplier: AAT BIOQUEST

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production.

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Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Pierce™

Supplier: Thermo Fisher Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

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ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

ReadiLink™ iFluor® 488 Oligo and ssDNA Labelling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

ReadiLink™ Rapid XFD555 Antibody Labelling Kit *XFD555 Same Structure to Alexa Fluor™ 555* (2×50 µg Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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Cell Explorer™ Live Cell Tracking Kit

Cell Explorer™ Live Cell Tracking Kit

Supplier: AAT BIOQUEST

The kit uses a weakly fluorescent dye that carries a cell-retaining moiety.

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Buccutite™ Poly-HRP Antibody Conjugation Kit

Buccutite™ Poly-HRP Antibody Conjugation Kit

Supplier: AAT BIOQUEST

Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

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Crosslink immunoprecipitation (IP) kit, Pierce™

Crosslink immunoprecipitation (IP) kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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Antibody labelling kits, DyLight™

Antibody labelling kits, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

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Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Speciality dyes, DyLight™

Speciality dyes, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

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NHS ester quenchers, DyLight™

NHS ester quenchers, DyLight™

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

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Biotin-NHS

Supplier: Merck Millipore (Oncogene)

Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

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PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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AZOCOLL™ substrate, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.

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