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You searched for: Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

ReadiLink™ Rapid mFluor™ Red 700 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

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iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate

iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate

Supplier: AAT BIOQUEST

iFluor® 790 labelled WGA conjugate exhibits a distinct near-infrared fluorescence characteristic, emitting maximally at 812 nm.

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ReadiLink™ iFluor® 555 Oligo and ssDNA Labelling Kit

ReadiLink™ iFluor® 555 Oligo and ssDNA Labelling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

PE-Cy5 is a popular colour used in flow cytometry.

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Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.

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Magnetic RNA-protein pull-down kit, Pierce™

Magnetic RNA-protein pull-down kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Magnetic RNA-Protein Pull-Down Kit is a streamlined, robust method to enrich protein-RNA interactions using end-labelled RNA as the bait.

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RNA 3' End desthiobiotinylation kit

RNA 3' End desthiobiotinylation kit

Supplier: Thermo Fisher Scientific

Pierce™ RNA 3' End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

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Buccutite™ Rapid Crosslinked APC Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid crosslinked APC antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE and iFluor® tandem dyes.

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Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm.

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ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

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ReadiLink™ Rapid Cy7 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy7.

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ReadiLink™ Rapid Cy3 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy3.

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Fluorescent dye removal columns, Pierce™

Fluorescent dye removal columns, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-iFluor® 700 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

mFluor™ dyes are developed for flow cytometry-focused applications.

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Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 594 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 350 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ BSA Conjugation Kit (2 Reactions)

ReadiLink™ BSA Conjugation Kit (2 Reactions)

Supplier: AAT BIOQUEST

Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

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Long stokes shift dyes, DyLight™, Pierce™

Long stokes shift dyes, DyLight™, Pierce™

Supplier: Thermo Fisher Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 to 145 nm in excitation/emission wavelengths. The DyLight™ LS dyes provide multicolour detection using a single excitation source in applications including flow cytometry, conjugation, DNA-sequencing, microscopy and fluorescence in situ hybridization (FISH). These dyes contain N-hydroxysuccinimide (NHS) esters, the most commonly used reactive group for labeling proteins. NHS esters react with primary amines on proteins and other molecules, forming a stable, covalent amide bond and releasing the NHS group.

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ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Pierce™ Deuterium-labelled Cross Linkers

Pierce™ Deuterium-labelled Cross Linkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Supplier: AAT BIOQUEST

XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher.

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ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Carbohydrate reactive biotin reagents, HOOK™

Supplier: G-Biosciences

HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.

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Streptavidin, hydrazide-activated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Supplier: Thermo Fisher Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

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Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ NHS-Fluorescein antibody labeling kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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