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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible* (2 Labelings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.

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Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm.

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ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.

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ReadiLink™ Rapid Cy7 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy7.

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ReadiLink™ Rapid Cy3 Antibody Labelling Kit

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy3.

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Fluorescent dye removal columns, Pierce™

Fluorescent dye removal columns, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

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Magnetic RNA-protein pull-down kit, Pierce™

Magnetic RNA-protein pull-down kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Magnetic RNA-Protein Pull-Down Kit is a streamlined, robust method to enrich protein-RNA interactions using end-labelled RNA as the bait.

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RNA 3' End desthiobiotinylation kit

RNA 3' End desthiobiotinylation kit

Supplier: Thermo Fisher Scientific

Pierce™ RNA 3' End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

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Buccutite™ Rapid Crosslinked APC Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid crosslinked APC antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE and iFluor® tandem dyes.

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ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)

Supplier: AAT BIOQUEST

mFluor™ dyes are developed for flow cytometry-focused applications.

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ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Supplier: AAT BIOQUEST

The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).

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Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 594 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*

Supplier: AAT BIOQUEST

Helixyte™ iFluor® 350 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.

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ReadiLink™ BSA Conjugation Kit (2 Reactions)

ReadiLink™ BSA Conjugation Kit (2 Reactions)

Supplier: AAT BIOQUEST

Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

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Pierce™ Deuterium-labelled Cross Linkers

Pierce™ Deuterium-labelled Cross Linkers

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

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Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*

Supplier: AAT BIOQUEST

XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher.

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ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kit

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-iFluor® 700 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

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Streptavidin, hydrazide-activated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.

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Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Pierce™ NHS-Fluorescein antibody labeling kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ NHS-Fluorescein antibody labeling kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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ReadiLink™ Rapid XFD594 Antibody Labelling Kit 'XFD594 Same Structure to Alexa Fluor™ 594' (2×50 µg Labellings)

ReadiLink™ Rapid XFD594 Antibody Labelling Kit 'XFD594 Same Structure to Alexa Fluor™ 594' (2×50 µg Labellings)

Supplier: AAT BIOQUEST

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents

Supplier: Thermo Fisher Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

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Avidin, horseradish peroxidase (HRP) conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

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ReadiLink™ KLH Conjugation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. This ReadiLink™ KLH conjugation kit is primarily optimised for the simple preparation of hapten-carrier conjugates for immunisation and antibody production.

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HIS Lite™ Cy3 Bis NTA Chelator

HIS Lite™ Cy3 Bis NTA Chelator

Supplier: AAT BIOQUEST

Cy3 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.

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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)

Supplier: AAT BIOQUEST

XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)

Supplier: AAT BIOQUEST

XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).

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