Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, PBS solution 10X concentrate (phosphate buffered saline)
Supplier: Roth Carl
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
Supplier: Serva
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Ammonium Chloride Solution
Supplier: Stemcell Technologies
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer)
Supplier: Serva
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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Buffer, TRIS solution pH 8,8 (1 mol/L)
Supplier: Serva
Cloning Buffer, TRIS buffer solution pH 8,8 (1 mol/l)
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EasySep™ Red Blood Cell Lysis Buffer
Supplier: Stemcell Technologies
EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Serva
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Molecular Biology Grade
Supplier: Serva
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Buffer, sodium acetate buffer solution pH 7 (3 mol/L)
Supplier: Serva
Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)
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