Buffers

Supplier: Honeywell Chemicals

These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS) with EDTA, 5X, Liquid

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Anode buffer (50X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, pH value: 3,0, Odourless, Liquid

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Corning

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.